| # | University | City | World rank | In-country | Citations |
|---|---|---|---|---|---|
| 1 | Universidad del Valle de Guatemala | not ranked | #1 | 71,229 | |
| 2 | Universidad de San Carlos de Guatemala | not ranked | #2 | 58,079 | |
| 3 | Universidad Francisco Marroquín | not ranked | #4 | 17,248 | |
| 4 | Universidad Galileo | not ranked | #5 | 4,862 | |
| 5 | Universidad del Istmo | not ranked | #6 | 3,057 | |
| 6 | Universidad Rafael Landívar | not ranked | #7 | 2,571 | |
| 7 | Universidad Mariano Gálvez | not ranked | #8 | 1,825 | |
| 8 | Centro Universitario de Occidente | not ranked | #9 | 1,386 | |
| 9 | Centro Universitario Ciudad Vieja | not ranked | #10 | 741 | |
| 10 | Universidad Rural de Guatemala | not ranked | #11 | 100 |
What the two columns mean
In-country is the position the ranking record gives the university among those in its own country, and it is often the more useful number: a university ranked 480th in the world may still be third at home. It is carried over as published, so it does not always follow the order of the other two columns. World rank is the university's QS World University Rankings position. Citations are the number of times work published by the university has been cited, as recorded in OpenAlex; it is the measure used to order universities that QS does not rank at all.
The two do not always agree. A large research university can out-cite a smaller one that sits above it in QS, because QS weighs reputation surveys and staff ratios as well as research.